Cat. No.
MABL-1326
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Rat, Swine, Zebrafish, Human, Mouse
Clone No.
RKSE60
From
Recombinant Antibody
Specificity
The original antibody reacts exclusively with Cytokeratin 10 which is present in Keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas.
Alternative Names
K10; CK-10; Keratin K10; Keratin type I cytoskeletal 10; Cytokeratin-10; Keratin-10
UniProt
P13645
Immunogen
The original antibody was generated by immunizing a BALB/c mouse with Cytokeratins from the Human epidermis.
Application Notes
The original antibody specifically reacted with keratinizing stratified epithelia and squamous cell carcinomas derived from these by IHC (Ramaekers et al. 1983; PMID： 6350235). The antibody stained cytokeratin 10 from cultured human keratinocytes by immunohistochemistry (Muijen et al, 1987; PMID： 2442018). This antibody was used for detection of cytokeratin 10 in psoriatic skin and normal skin by flow cytometry (van Erp et al., 1989; PMID： 2479273). The antibody detected cytokeratin 10 from human foreskin keratin preparation, by western blot analysis (Ramaekers et al., 1990; PMID： 1690512).
Antibody First Published
Ramaekers et al. Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumors： an overview Histochem J. 1983 Jul;15(7)：691-713. PMID：6350235
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
