Cat. No.
MABL-1329
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4D2
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 410-432 (TIVEEVQDGKVISSREQVHQTTR) of human cytokeratin 17. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 17 regulates the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1).
Alternative Names
CK17; CK-17; KRT17; K17; Keratin; type I cytoskeletal 17; Cytokeratin-17; Keratin-17; CGMCC NO 20762; E3
UniProt
Q04695
Immunogen
The original antibody was generated by immunizing female BALB/c mice with an immunogenic polypeptide containing amino acids 410-432 (TIVEEVQDGKVISSREQVHQTTR) of human cytokeratin 17 conjugated with KLH.
Application Notes
The binding characterization of this antibody to human cytokeratin 8 was done using ELISA. The affinity constant for the original IgG3 antibody was calculated to be 9.6 × 10^(8) M^(-1). This antibody is similar to commercially available anti-Cytokeratin 17 antibody E3 in staining various human tissues. This antibody can be used in the immunohistochemical analysis of esophageal squamous cell carcinoma (CN113278070).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
