Cat. No.
MABL-1335
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HH156
From
Recombinant Antibody
Specificity
This antibody is specific for extracellular domain of the human cytokeratin 18 containing amino acids 'LEDGEDFNLGDALD'. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 18 is widely expressed in human monolayer epithelial tissues, and generally appears in pairs with CK8. CK18 is a serum marker for a variety of tumor diagnosis, as well as a serum marker for liver injury. It is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, it plays a role in filament reorganization. It is also involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Alternative Names
CK18; CK-18; CK 18; KRT18; CYK18; Keratin type I cytoskeletal 18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein
UniProt
P05783
Immunogen
The original antibody was generated by immunizing BALB/c mice with human cytokeratin 18 extracellular domain polypeptide 'LEDGEDFNLGDALD'. Later on the humanized version of this antibody was generated by grafting the CDRs of the original mouse antibody E156 onto human framework regions.
Application Notes
This antibody can bind human cytokeratin 18 in an ELISA. The original IgG1 version of this antibody is reported to bind human cytokeratin 18 with a binding affinity of 32.8 pM.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
