Cat. No.
MABL-1343
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Feline, Hamster, Rabbit, Rat, Swine, Human, Mouse
Clone No.
RCK102
From
Recombinant Antibody
Specificity
The original antibody binds to Cytokeratin 5 and 8.
Alternative Names
K5; CK-8; Keratin K5 + K8; Keratin, type II cytoskeletal 8; Cytokeratin-8; Keratin-8; K8; Type-II keratin Kb8; Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; Type-II keratin Kb5
UniProt
P05787; P13647
Immunogen
The original antibody was generated by immunizing a mouse with cytokeratins from a Human lung cancer cell line (MR21).
Application Notes
The antibody recognized keratins 5 and 8, and as a result stained virtually all epithelial tissues. This antibody was used for immunohistochemistry on classic SCLC as well as the adenocarcinoma cell lines (Broers et al., 1986; PMID: 2433295). Further, endocervical columnar cells, cervical immature squamous metaplasia, cervical CIN I, CIN II and CIN III reacted with the antibody in immunohistochemistry (Smedts et al., 1990; PMID: 1690513). The antibody detected keratins 5 and 8 by western blot analysis on T 24, HeLa and KB cells (Ramaekers et al., 1987; PMID: 2436934).
Antibody First Published
Broers et al. Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies J Cell Sci. 1986 Jul;83:37-60. doi: 10.1242/jcs.83.1.37. PMID: 2433295
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
