Cat. No.
MABL-1387
Application
crystallization, RIA, ELISA
Isotype
Engineer antibody
Species Reactivity
Digitalis lanata
Clone No.
26 / 10
From
Recombinant Antibody
Specificity
The antibody binds digoxin with an affinity (Kd) of 9 nM.
Alternative Names
Digitalis
UniProt
Immunogen
Spleen cells from A/J mice immunized with digoxin coupled to human serum albumin (Dig- HSA).
Application Notes
The antibody is specific for digoxin, a cardiac glycoside derived from the foxglove plant. Digoxin is administered for the therapeutic treatment of heart conditions such as atrial fibrillation, atrial flutter and heart failure, an acts by inhibiting the Na+/K+ ATPase. However, the drug has a very narrow therapeutic index and can cause toxic side effects. The Fab fragment prepared from 26-10 that neutralises the activity of digoxin and can be used as an antidote in case of overdose of digitalis. Anti- digoxin monoclonal antibodies can also be used to measure serum levels of the drug as a guide to therapy, and are useful in the investigation of the kinetics of interaction between antibody and antigen (Mudgett- Hunter et al., 1985; PMID: 4033667). The structure of the Fab fragment of the antibody was determined by XRD crystallography (Jeffrey et al., 1993; PMID: 8234291).
Antibody First Published
Mudgett-Hunter M, Anderson W, Haber E, Margolies MN. Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies. Mol Immunol. 1985 Apr;22(4):477-88. PMID: 4033667
Note on publication
Describes the generation of high-affinity monoclonal anti-digoxin antibodies using the hybridoma technology. The antibodies can be useful for the study of structure-function relationships between antibody binding site and antigenic determinants.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
