Cat. No.
MABL-1452
Application
IP, WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
4A2
From
Recombinant Antibody
Specificity
This antibody recognizes E cadherin which is one of the most important molecules in cell-cell adhesion in epithelial tissues. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. The 4A2 monoclonal antibody recognizes the cytoplasmic domain of E- cadherin. The epitope has been mapped to residues 757–778.
Alternative Names
Cadherin-1; CD324; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin
UniProt
P12830
Immunogen
The original mouse IgG1 version of this antibody was raised by immunizing mice with the recombinant maltose binding protein fused to full length human E-Cadherin.
Application Notes
4A2 antibody has been widely used for identification and analysis of E cadherin. The original mouse IgG1 version was used to detect the intracellular domain of E cadherin in A431 or A431-E- cad-M cells (Nieman et al., 1999; pmid: 10212155). It was also demonstrated that this antibody can detect E cadherin on Western blots (Li et al., 2019; pmid: 31245295). Similarly, 4A2 antibody was also utilized to immunoprecipitate E cadherin from LNCaP and C4-2 cell extracts (Jaggi et al., 2005; pmid: 15695390).
Antibody First Published
Nieman et al. Mechanism of extracellular domain-deleted dominant negative cadherins J Cell Sci. 1999 May;112 ( Pt 10):1621-32. PMID:10212155
Note on publication
This article describes the characterization of the 4A2 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
