Cat. No.
MABL-1453
Application
agonist, ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
56-4
From
Recombinant Antibody
Specificity
This antibody binds mouse E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis.
Alternative Names
CD324; ARC-1; Cdh1; Cadherin-1; Cadherin E; E-Cadherin; Uvomorulin
UniProt
P09803
Immunogen
The original antibody was generated by immunizing rabbits with purified extracellular domain of mouse E-cadherin.
Application Notes
The binding of this antibody to mouse E-cadherin results in its activation. The initial binding characterization of this antibody to mouse E-cadherin was done using ELISA. This antibody was used to study the effect of activation of mouse E-cadherin on the growth and metastasis of a spontaneous breast cancer mouse model. Female MMTV-PyMT and control mice treated with 5 mg/kg of this antibody developed tumors in all 10 mammary glands. Also the number of metastasis nodules was significantly reduced with treatment with 56-4 activating antibody (PMID: 32127478; WO2020243616).
Antibody First Published
Young Na et al. The functional activity of E-cadherin controls tumor cell metastasis at multiple steps. Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):5931-5937. PMID:32127478
Note on publication
The paper describes the use of activating antibodies to determine the role of E- cadherin in regulation of metastasis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
