Cat. No.
MABL-1454
Application
NTRL, WB, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
DENV4
Clone No.
DV22
From
Recombinant Antibody
Specificity
This antibody recognises domain I and II of the envelope (E) protein of DENV4 only, shown by ELISA and Western blot.
Alternative Names
DENV EI/II; Dengue envelope protein domain I/II; E protein domain I/II; DENV EDI/II; EDI/II; DV 22; DV-22
UniProt
Immunogen
Antibody DV22 was isolated from peripheral blood mononuclear cells collected from a patient diagnosed with dengue infection.
Application Notes
DV22 has been used in cell-based assays and was shown to exhibit neutralisation and antibody-dependent enhancing activity using Vero and K562 cells (Beltramello et al, 2010). This antibody has also been characterised as EDI/II-specific by ELISA and Western blot. When generated as a LALA variant and supplied as a cocktail with two other anti-DENV mAbs, DV22 showed a complete lack of antibody- dependent enhancement (ADE) activity, whilst retaining the ability to neutralise DENV infection.
Antibody First Published
Beltramello et al. The human immune response to Dengue virus is dominated by highly cross-reactive antibodies endowed with neutralizing and enhancing activity. Cell Host Microbe. 2010 Sep 16;8(3):271-83. PMID:20833378
Note on publication
Describes the characterisation of a large panel of anti-DENV mAbs isolated from DENV-infected patients.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
