Anti-EpCAM mAbs [G8.8](MABL-1501)

MABL-1501

IP, FC, IF, IHC

Engineer antibody

Human, Mouse

G8.8

Recombinant Antibody

This antibody is specific for human and mouse EpCam. EpCam may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1

P16422

The original version of this antibody was raised by immunizing a rat with glycoconjugates isolated from TE-71 cells.

To research scarring in humans, immunofluorescence was preformed on human skin and scar biopsies using the rat version of this antibody (Jiang et al, 2020; pmid:33159076). To show that PDCs expressing the established surface and cancer stem cell marker EpCAM give rise to HCC in inflamed liver, immunohistochemistry was preformed on mouse liver cells using the rat version of this antibody (Matsumotot et al, 2017; pmid:28951464). To show that major facilitator super family domain containing 2a (Mfsd2a), previously known to maintain blood–brain barrier function, is a periportal zonation marker, immunofluorescence was preformed on liver cells from Mfsd2a-CreER mice using the rat version of this antibody (Pu et al, 2016; pmid:27857132). To research the expression of EpCam, immunohistochemistry was preformed on mosue thymus cells using the rat version of this antibody. Further, the antigen of this antibody was immunoprecipitated using the rat version of this antibody bound to sepharose beads (Farr et al, 1991; pmid:2016514). Epithelial cell adhesion molecule expression in A2C12, A549, and Caco-2 cells was determined by flow cytometry using the rat version of this antibody. Furthermore, cell proliferation of A2C12, A549, and Caco-2 cells under the influence of the rat version of this antibody was determined. A significant increase in cell proliferation was observed (Maaser and Borlak, 2008; pmid:19002182). To look at the expression patterns of EpCam., immunohistochemistry was preformed on both human and murine tissue using this antibody. The tissues used were pancreas, prostate, brain and endothelium tissue (Amann et al, 2008; pmid:18172306). To study the expression of EpCam at an early stage of liver development immunohistochemistry was preformed on whole mice embryos and fetal liver using the rat version of this antibody (Tanaka et al, 2009; pmid:19527784). To establish a system to purify spermatogonial stem cells, the rat version of this antibody was used for flow cytometry on testis cells from 5- to 10 week old male mice (Kanatsu-Shinohara et al, 2011; pmid:21858196).

Farr et al. Epithelial Heterogeneity in the Murine Thymus: A Cell Surface Glycoprotein Expressed by Subcapsular and Medullary Epithelium J Histochem Cytochem. 1991 May;39(5):645-53. PMID:2016514

A monoclonal antibody (MAb), G8.8, was raised against glycoconjugates isolated from a cloned line of murine medullary thymic epithelial cells.

100 μg Purified antibody.

1 mg/ml.

Protein A affinity purified

PBS with 0.02% Proclin 300.

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.