Cat. No.
MABL-1552
Application
functional assay, inhibit, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KM33
From
Recombinant Antibody
Specificity
This antibody binds the C1 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Alternative Names
AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
UniProt
P00451
Immunogen
The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.
Application Notes
The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 97 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 0.1 nM. It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII, although its epitope is located outside region R1803-K1818 (PMID: 11159524). It was reported that the presence of this antibody completely inhibited FVIII endocytosis by both monocyte and bone marrow derived mouse dendritic cells (PMID: 21962992). It was reported that this antibody inhibits FVIII activity and interactions with its physiological ligands, von Willebrand factor, phospholipids and catabolic receptors in vitro (PMID: 11159524; Limburg V et al., 2005).
Antibody First Published
van den Brink et al. Multiple VH genes are used to assemble human antibodies directed toward the A3-C1 domains of factor VIII. Blood. 2001 Feb 15;97(4):966-72. PMID:11159524
Note on publication
Describes the generation and epitope mapping of antibodies against A3 domain of factor VIII.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
