Cat. No.
MABL-1553
Application
functional assay, inhibit, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KM41
From
Recombinant Antibody
Specificity
This antibody binds and epitope between amino acid R1803-K1818 of the A3-C1 domains of the human factor VIII, which also contain binding site for factor IXa. Factor VIII is a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Alternative Names
AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
UniProt
P00451
Immunogen
The original antibody was isolated from a human phage display library generated from the peripheral blood of an inhibitor patient and panning the library against factor VIII light chain.
Application Notes
The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 63 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 1.6 nM (PMID: 11159524). It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII. In view of the similar epitope specificity of scFv KM41 and CLB-CAg A (antibody which binds a synthetic peptide comprising residues R1803-K1818), it is likely that scFv KM41 competes with factor IXa for binding to the factor VIII light chain (PMID: 11159524).
Antibody First Published
van den Brink et al. Multiple VH genes are used to assemble human antibodies directed toward the A3-C1 domains of factor VIII. Blood. 2001 Feb 15;97(4):966-72. PMID:11159524
Note on publication
Describes the generation and epitope mapping of antibodies against A3 domain of factor VIII.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
