Cat. No.
MABL-1554
Application
column chromatography, Depletion, WB, Block
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
RFF-VIII:c/8
From
Recombinant Antibody
Specificity
This antibody is specific for human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder haemophilia A.
Alternative Names
AHF; Antihemophilic factor; coagulation factor VIII; procoagulant component; coagulation factor VIIIc; DXS1253E; F8C; factor VIII F8B; FVIII; HEMAF8B; Procoagulant component
UniProt
P00451
Immunogen
This antibody was raised by immunising BALB/C mice with highly purified procoagulant factor VIII (VIII: C). Spleen cells were then isolated from immunised mice, and fused with P3 NSI mouse myeloma cells to generate hybridomas.
Application Notes
This antibody is a potent coagulation inhibitor (Rotblat, 1983).This antibody has been employed to produce factor VIII-deficient plasma by immunodepletion (Takase et al, 1987); purified monoclonal antibodies were bound to Sepharose columns, which were used to reduce the factor VII content of plasma through column chromatography. This antibody does not bind antigens if CRM negative haemophilic plasma, which lacks factor VIII, is used in column chromatography (Rotblat et al, 1983). This antibody has also been used in Western blotting to identify factor VIII (Rotblat et al, 1985)
Antibody First Published
Rotblat et al Monoclonal antibodies to human procoagulant factor VIII. Journal of Laboratory and Clinical Medicine, June 1983 PMID:6403638
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
