Cat. No.
MABL-1555
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
YK3.3.38
From
Recombinant Antibody
Specificity
This antibody is specific for an epitope 'RPLYSRRLPKGVKHLKDFPILPGEI' (residues 389 to 510) in the A2 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder hemophilia A.
Alternative Names
AHF; FVIII; Factor 8; anti-hemophilic factor; Coagulation factor VIII; procoagulant component
UniProt
P00451
Immunogen
The original antibody was isolated from a scFv phage display library generated using the peripheral blood lymphocytes from a hemophilia patient.
Application Notes
This antibody can bind human blood coagulation factor VIII in an ELISA. It was reported that this antibody potently inhibits the coagulation activity of factor VIII in vitro at a concentration of 10 ng/ml. This antibody does not inactivate FVIII bound with vWF but inactivated FVIII free from vWF (US20040120951).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
