Cat. No.
MABL-1557
Application
column chromatography, immunoradiometric assay, Block
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
RFF-VIII:c/5
From
Recombinant Antibody
Specificity
This antibody is specific for a C-terminal site on human coagulation factor VIII
Alternative Names
AHF; Antihemophilic factor; coagulation factor VIII; procoagulant component; coagulation factor VIIIc; DXS1253E; F8Ccoagulation factor VIII; factor VIII F8B; FVIII; HEMAF8B; Procoagulant component
UniProt
P00451
Immunogen
This antibody was raised by immunising BALB/C mice with highly purified procoagulant factor VIII (VIII: C)
Application Notes
This antibody is a coagulation inhibitor, though displays only weak anticoagulant activity (Rotblat et al, 1983). It has been used as the solid-phase immunoadsorbent in a two-site immunoradiometric assay of Factor VIII:c (Rotblat et al, 1983).When bound to Sepharose columns, this antibody has been used to deplete factor VII:c from plasma through column chromatography (Takase et al, 1987).
Antibody First Published
Rotblat et al. Monoclonal antibodies to human procoagulant factor VIII. Journal of Laboratory and Clinical Medicine, June 1983 PMID:6403638
Note on publication
Describes the original generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
