Cat. No.
MABL-1559
Application
IP, WB, IF
Isotype
Engineer antibody
Species Reactivity
Chicken
Clone No.
5B9
From
Recombinant Antibody
Specificity
This antibody is specific for chicken tensin. Tensin may be involved in cell migration, fibrillar adhesion formation, cartilage development and in linking signal transduction pathways to the cytoskeleton.
Alternative Names
tensin; FAK; p210
UniProt
Q04205
Immunogen
The mouse version of this antibody was generated by immunizing mice with a mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src, and recognizes FAK.
Application Notes
To study bacterial invasion. Lysates from 5637 cells infected with a bacterial suspension were immunoblotted using the mouse version of this antibody (Martinez et al, 2000; pmid:10856226). To study fibronectin matrix assembly. Western blot and immunoprecipitation was preformed on collagen-adherent FN-null cell monolayers. Furthermore FN-null MEFs were used for immunofluorescence (Lefort et al, 2011; pmid:21084302). Lysates from CE cells were both immunoprecipitated and blotted using the mouse version of this antibody. This was done to determine the wether CAKβ shares properties with pp125FAK (Schaller and Sasaki, 1997; pmid:9312150).
Antibody First Published
Schaller and Sasaki. Differential signaling by the focal adhesion kinase and cell adhesion kinase beta J Biol Chem. 1997 Oct 3;272(40):25319-25. PMID:9312150
Note on publication
pp125(FAK) and CAKbeta/Pyk2/CadTK/RAFTK are related protein-tyrosine kinases. It is therefore of interest whether CAKbeta shares some of the properties of pp125(FAK). Using recombinant glutathione S-transferase fusion proteins, it is shown that the C-terminal domains of both proteins bind paxillin in vitro.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
