Cat. No.
MABL-1571
Application
Blocking, MST, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
E. coli
Clone No.
NbFedF9
From
Recombinant Antibody
Specificity
Specific to the N terminal lectin domain as this is the domain represented in the 15-165 fragment. It has an affinity for FedF(15–165) of around 1.5 nM (Moones et al, 2014).
Alternative Names
F18 fimbrial adhesin AC; F18 fimbrial adhesin
UniProt
Q47212
Immunogen
A Llama was immunised 6 times with purified FedF residues 15-165 over a period of 6 weeks. Lymphocytes were extracted and used to prepare cDNA which was used as a template fthe VHH. These were then transfected into E. coli and the antibodies selected by phage display. This was then subjected to ELISA to confirm the specificity of the antibodies.
Application Notes
It has been shown that this antibody can inhibit the binding of FedF to piglent enterocytes. The structure of this antibody binding to FedF has been resolved to a resolution of 1.5A. This shows that the mechanism of inhibition of FedF binding to epithelial cell surfaces is by competing at the carbohydrate binding site. Both MST and SPR have been used to analyse binding affinities (Moones et al, 2014).
Antibody First Published
Moonens et al Nanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli PLoS One. 2014; 9(12): e114691. PMID: 25502211
Note on publication
Discusses the generation of this antibody, its crystallisation and the method of netralisation of FedF
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
