Cat. No.
MABL-1578
Application
IP, WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1C9
From
Recombinant Antibody
Specificity
This antibody binds the human FGF-1.
Alternative Names
FGF-1; aFGF; ECGF; HBGF-1; Fibroblast growth factor 1; Acidic fibroblast growth factor; Endothelial cell growth factor; Heparin-binding growth factor 1
UniProt
P05230
Immunogen
The original antibody was generated by immunizing mice with human FGF-1.
Application Notes
FGF-1 plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Studies have shown that FGF-1 is overexpressed in the early stages of several kinds of cancer. Functional analysis showed that the overexpressed scFv1C9 in MCF- 7 cells targeted endogenous FGF-1 and prevented the translocation of FGF-1 into the nucleus, resulting in the blockade of the intracrine pathway of FGF-1, which caused the G1 arrest by p21 up-regulation. Binding of scFv1C9 to FGF-1 was confirmed with ELISA. The antigen-binding ability of scFv1C9 with endogenous FGF- 1 was confirmed in vivo by using of an immunoprecipitation assay. FGF-1 immunohistochemical staining of breast ductal carcinoma tissues was used to to demonstrate that FGF-1 was increasingly expressed in breast cancer tissues.(PMID:21360642).
Antibody First Published
Shi et al. A Novel Single-Chain Variable Fragment Antibody Against FGF-1 Inhibits the Growth of Breast Carcinoma Cells by Blocking the Intracrine Pathway of FGF-1. IUBMB Life (2011); 63(2); 129–137. PMID:21360642
Note on publication
Describes the role of anti-FGF1 antibody in blocking the intracrine pathway of FGF-1 and inhibiting growth of breast carcinoma cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
