Cat. No.
MABL-1605
Application
in vitro, in vivo, inhibition, ELISA
Isotype
Engineer antibody
Species Reactivity
Salmonella enterica
Clone No.
0A07
From
Recombinant Antibody
Specificity
This antibody is specific for the flagellin protein (FliC) of Salmonella enterica, which plays a pivotal role in bacterial motility and pathogenicity. FliC is the main structural component of the bacterial flagella in Salmonella enterica, tail-like structures essential for bacterial movement and invasion of host tissues. Targeting FliC can potentially neutralize the pathogen by hindering its motility, thus preventing its invasion of host cells. Additionally, antibodies against FliC may facilitate bacterial recognition and elimination by the host immune system, aiding in the clearance of the bacteria from the body.
Alternative Names
FliC; AbiSeO7
UniProt
Immunogen
The original antibody was generated by immunizing alpacas with heat-inactivated Salmonella cells.
Application Notes
The original version of this antibody (alpaca VHH) exhibited the highest inhibition of intracellular S. enterica (strain Hadar 5643) growth out of all of the tested antibodies. In vitro, the antibody was evaluated for its ability to disrupt Salmonella colonization in chicken intestinal cultures, resulting in a significant 7.5-log reduction in Salmonella genome copy numbers in ileum sections. In vivo, it was tested for its protective efficacy in broiler chickens challenged with Salmonella enterica via oral gavage. The antibody significantly decreased Salmonella levels by 0.7 log10 in both jejunum and ileum compared to the control group when administered at three different time points post-challenge (US10421806B2).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
