Cat. No.
MABL-1639
Application
flow cytometry, in vivo, neutralization, SPR, X-ray crystallography, ELISA
Isotype
Engineer antibody
Species Reactivity
Hendra henipavirus, Nipah virus
Clone No.
HENV-26
From
Recombinant Antibody
Specificity
The antibody is specific for RBP of HeV and NiV. The antibody epitope overlaps with the receptor binding sites of RBP.
Alternative Names
RBP; Glycoprotein; Glycoprotein G; Attachment glycoprotein; Receptor binding protein;
UniProt
Q9IH62; F4YH71; H6V875; O89343
Immunogen
The original antibody was isolated from an immune human individual.
Application Notes
The specificity of the original format of the antibody for recombinant RBP head domain proteins from HeV, NiVM, or NiVB was confirmed by ELISA analysis (EC50 = 0.14,0.09 and 0.07 ug/mL respectively). The antibody could neutralize HeV, NiVB and NiVM in In vitro neutralization assays (IC50= 0.07, 0.03 and 0.04 ug/mL respectively). The antibody could bind to the full-length RBPs by flow cytometry. The EC50 values for binding of the antibody ranged from 325 to 343 ng/mL for binding to HeV, NiVM, or NiVB. The kinetics of binding of the antibody to RBPs was measured on a biosensor to determine affinity (KD= 2.9, 2.2, or 1.0 nM for HeV, NiVM, or NiVB, respectively). The crystal structures of Fab fragment of the antibody in complex with both HeV-RBP and NiV-RBP were determined. The antibody protected ferrets in lethal models of infection with NiV (Dong et al., 2020; PMID： 33306954).
Antibody First Published
Dong et al. Potent Henipavirus Neutralization by Antibodies Recognizing Diverse Sites on Hendra and Nipah Virus Receptor Binding Protein Cell. 2020 Dec 10;183(6):1536-1550.e17. doi: 10.1016/j.cell.2020.11.023. PMID:33306954
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
