Cat. No.
MABL-1707
Application
Blocking, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Dog, Rat, Human, Mouse
Clone No.
Gipg013
From
Recombinant Antibody
Specificity
The original has a Ki value of 7 nM and a Kd value of 6.8 nM for human GIPr.
Alternative Names
GIPr
UniProt
Q8TDV5
Immunogen
Human GIPr.
Application Notes
This antibody binds to GIPr, a GPCR expressed on pancreatic beta-cells, where activation leads to the release of insulin. Endogeonous ligands for the receptor include oleylethanolamide and lysophosphatidylcholine. The antibody acts as a competitive antagonist. In rat islets, the antibody inhibits GIP-induced secretion of insulin by up to 81%.
Antibody First Published
Ravn et al. Structural and pharmacological characterization of novel potent and selective monoclonal antibody antagonists of glucose-dependent insulinotropic polypeptide receptor. Journal of Biological Chemistry 2013; 288(27)：19760-19772 PMID：23689510
Note on publication
Describes the generation of a monoclonal antibody against GIP and subsequent crystallisation as well as inhibition studies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
