Cat. No.
MABL-1765
Application
WB, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
KM-2
From
Recombinant Antibody
Specificity
KM-2 antibody recognizes the N-terminal peptides 1-20 of histone H2A and 1-29 of histone H4 which have a large sequence homology (van Bruggen et al., 1997; pmid: 9027774). Histone H4 acetylation on lysines 8, 12 and 16 significantly increases the antibody's binding while the acetylation at K5 decreases it (Dieker et al., 2007; pmid: 17530637).
Alternative Names
histone 4; nucleosome; H4K8Ac; H4K12Ac; H4K16Ac; acetyl-Histone H4; Histone H4 acetylated at lysine; KM2
UniProt
Immunogen
KM-2 antibody was derived from an MRL/lpr lupus mouse.
Application Notes
The antibody KM-2 shows significant increase in activity with histone H4 when it is acethylated on lysines 8, 12 and 16 which are hyperacethylated in conditions, such as lupus (SLE); and thus KM-2 can be used in the detection of the apoptosis-induced histone modifications characteristic of lupus and in the analysis of its pathogenesis (Dieker et al., 2007; pmid: 17530637). This antibody was successfully used to identify both Histone H4 as well as H2A in the extracts of Jurkat cells via Western blot, showing considerable increase in activity in cells with induced apoptosis leading to hyperacethylation (Dieker et al., 2007; pmid: 17530637). Similar results were achieved for the immunofluorescence staining of Jurkat cells. The enhanced reactivity of KM‐2 with apoptotic H4 and apoptotic H2A was not Jurkat cell specific and was also observed for the monocytic U937 cell line which indicated its general applicability (Dieker et al., 2007; pmid: 17530637). Analogous reselts were also achieved during the ELISA analysis of plasma samples of (diseased) MRL/lpr lupus mice, with the visisble activity enhancement in the sick, apoptotic specimens (Dieker et al., 2007; pmid: 17530637). Finally, KM-2 (KM2) antibody was used to identify histones in the immune deposits in kidney biopsy samples of DPGN (Diffuse proliferative glomerulonephritis) patients (van Bruggen et al., 1997; pmid: 9027774).
Antibody First Published
van Bruggen et al. Nucleosomes and histones are present in glomerular deposits in human lupus nephritis. Nephrol Dial Transplant. 1997 Jan;12(1):57-66. PMID:9027774
Note on publication
This article describes the characterization of the KM-2 (KM2) antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
