Cat. No.
MABL-1767
Application
BLI, ELISA
Isotype
Engineer antibody
Species Reactivity
H5N1 Influenza A Virus
Clone No.
310-18A3
From
Recombinant Antibody
Specificity
This antibody is specific for the H5N1 and H1N1 subtypes of influenza A virus. Influenza viruses are negative-sense, single-stranded, segmented RNA viruses.
Alternative Names
Hemagglutinin; Avian Influenza Virus; AIV; H5N1; Influenza virus
UniProt
A5A5M5
Immunogen
This antibody was raised by cloning antibody sequences form B cells isolated from people immunized with the H5 DNA vaccine.
Application Notes
The affinity of this antibody to H1/1999 HA was assessed. This was done by using the human version of this antibody for Bio-Layer Interferometry on H1/1999 HA. Further, an ELISA was preformed using the human version of this antibody on HA from influenza (Whittle et al, 2014; pmid:24501410).
Antibody First Published
Whittle et al. Flow cytometry reveals that H5N1 vaccination elicits cross- reactive stem-directed antibodies from multiple Ig heavy-chain lineages. J Virol. 2014 Apr;88(8):4047-57. PMID:24501410
Note on publication
Structure-based modification of HA to isolate HA-specific B cells by flow cytometry was used and characterize the features of HA stem antibodies (Abs) required for their development.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
