Cat. No.
MABL-1768
Application
crystallization, in vivo, inhibition, neutralization, ELISA
Isotype
Engineer antibody
Species Reactivity
H1N1 Influenza A virus
Clone No.
5J8
From
Recombinant Antibody
Specificity
This antibody is specific for the H1N1 influenza virus hemagglutinin protein. It recognizses a conserved non-linear epitope (residues 133, 137 and 222) on the globular head of H1N1 HA between the receptor-binding pocket and the Ca2 antigenic site. The antibody inhibits all tested H1N1 influenza strains from 1918 to 1977 (A/South Carolina/1/1918, A/swine/Iowa/15/1930, A/Weiss/1943, A/L3/1947, A/New Jersey/11/1976, A/USSR/92/1977) and the pandemic 2009 virus but not the seasonal H1N1 strains from 1999 or 2007.
Alternative Names
Hemagglutinin
UniProt
G8XMJ2
Immunogen
The original antibody was isolated from the peripheral blood mononuclear cells of a healthy 47-year-old human subject.
Application Notes
The crystal structure of the Fab fragment in complex with a bacterially expressed and refolded globular head domain from the hemagglutinin was determined (Hong et al., 2013; PMID: 24027321). The antibody was inhibitory in a hemagglutination inhibition activity against H1N1pdm2009-like virus. Neutralization capacity of the antibody was prooved by an MN-assay. The specificity of the antibody to the monomeric head as to the full HA-trimer was confirmed by ELISA (Aartse et al., 2021; PMID: 34358138).
Antibody First Published
Krause et al. A broadly neutralizing human monoclonal antibody that recognizes a conserved, novel epitope on the globular head of the influenza H1N1 virus hemagglutinin J Virol. 2011 Oct;85(20):10905-8. PMID:21849447
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
