Cat. No.
MABL-1805
Application
inhibition, IP, SPR, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
t1E4
From
Recombinant Antibody
Specificity
The antibody is specific for HGF. It can bind to the two chains and to the single chain HGF. It recognizes an epitope located within the K4-SP region.
Alternative Names
HGF; HPTA; Hepatopoietin-A; Scatter factor
UniProt
P14210
Immunogen
The original antibody was generated by immunizing MRL/lpr mice with tcK2SP(Xa).
Application Notes
The specificity of the original format of the antibody for HGF was confirmed by ELISA analysis. The authors investigated if the antibody has any effect on the HGF-Met signaling. Met activation was strongly inhibited by the antibody, with an IC50 value of ~0.15 μg/ml or 0.1nM. In particular, antibody caused dose dependent reduction of the HGF-induced phosphorylation levels of Met (Tyr1234/1235), Akt (Ser473), and ERK (Thr202 and Tyr204). The inhibitory action of the antibody was further evaluated by two cell-based assays. The effect of all antibody on the HGF-induced scattering of Madin-Darby canine kidney (MDCK) cells was evaluated, and confirmed that only it could strongly suppress the scattering behavior of the cells when added at 10 μg/ml. Furthermore, the antibody was able to inhibit the HGF-stimulated migration of human liver bile duct carcinoma cells with a similar concentration dependency as the inhibition of Met phosphorylation in EHMES-1 cells. The antibody immunoprecipitated both scHGF and tcHGF. The binding affinity of the antibody toward the HGF proteins was tested by SPR.
Antibody First Published
Umitsu et al. Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies. Sci Rep. 2016 Sep 9;6:33149. PMID:27608665
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
