Cat. No.
MABL-1902
Application
WB, IP, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1E8-C6-B4
From
Recombinant Antibody
Specificity
The antibody is specific for the C-terminus of cytokeratin 8, one member of a diverse group of proteins called intermediate filament proteins. These proteins are often expressed in pairs. Cytokeratin 8 pairs with Cytokeratin 18 to form a component of the cytoskeleton in simple epithelial cells. Cytokeratin 8 is present on the surface of intact hepatocytes, hepatocellular carcinoma cells, and breast cancer cells.
Alternative Names
K8; Cytokeratin 8; CK 8; 1E8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8; 1E8
UniProt
P05787
Immunogen
The original antibody was generated by immunizing mice against the C-terminus (amino acids 472-483) of human Cytokeratin 8.
Application Notes
The original antibody detected CK 8 by western blot from BT20 breast cancer cells. Immunofluorescence was performed on BT20 cells using this antibody, showing the carboxyl terminus of CK 8 was exposed on the surfaces of breast cancer cells. The Fab fragment of the antibody was used for inhibition experiments on breast cancer cell lines BT20, MDA-MB-157, MCF-7 and purified CK 8. Results showed a decreased plasminogen binding in all the experiments. The Fab fragment was used to study the role of cell-surface CK 8 in promoting the activation of plasminogen by t-PA in MCF-7 cells; preincubation of MCF-7 cells with the Fab fragment caused a concentration-dependent decrease in the ability of the cell cultures to promote plasminogen activation (Hembrough et al, 1996; pmid:8810346).
Antibody First Published
Hembrough et al. Cell-surface cytokeratin 8 is the major plasminogen receptor on breast cancer cells and is required for the accelerated activation of cell-associated plasminogen by tissue-type plasminogen activator J Biol Chem. 1996 Oct 11;271(41):25684-91. PMID:8810346
Note on publication
The paper describes the generation and characterization of an antibody against cytokeratin 8 for the purpose of defining its immunological, cellular, and biochemical characteristics.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
