Cat. No.
MABL-1904
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
rhesus macaque, Human, Cynomolgus Monkey
Clone No.
17F12
From
Recombinant Antibody
Specificity
This antibody binds to all four human IgG subtypes (IgG1, IgG2, IgG3 & IgG4). Some cross- reactivity with rabbit IgG, cynomolgus monkey IgG1 and rhesus macaque IgG1 has also been reported.
Alternative Names
Immunoglobulin G
UniProt
Immunogen
This antibody was raised against human IgG.
Application Notes
A Fab and an scFv fragment derived from this antibody, conjugated with bacterial alkaline phosphatase, have been used in sensitive enzyme immunoassays for the detection of human IgG (Carrier et al, 1995). Further, the antibody reacted with human IgG1, cynomolgus monkey IgG1 and rhesus macaque IgG1 by ELISA analysis, but it did not show any cross reactivity with cynomolgus monkey IgG4.
Antibody First Published
Carrier et al. Recombinant antibody-alkaline phosphatase conjugates for diagnosis of human IgGs: application to anti-HBsAg detection Journal of Immunological Methods, 1995, Vol.181(2), pp.177-186 PMID:7745247
Note on publication
Describes the derivation of an scFv and Fab from this antibody, and their use in a sandwich ELISA.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
