Cat. No.
MABL-1905
Application
ELISA, IF
Isotype
Engineer antibody
Species Reactivity
chimpanzee, mangabey, vervet, Human
Clone No.
8E 11
From
Recombinant Antibody
Specificity
This antibody reacts with human IgG1, IgG2, IgG3, and IgG4, as well as mangabey, vervet and chimpanzee IgG. It has minimal reactivity with rhesus macaque, cynomolgus monkey or pigtailed macaque IgG.
Alternative Names
Human immunoglobulin G
UniProt
Immunogen
This antibody was raised by immunising mice with baboon IgG and screening hybridomas against IgG of different primate species.
Application Notes
The ability of this antibody to distinguish between human and macaque IgG has been confirmed in ELISA analysis. As a result of this specificity, this antibody has been used in immunofluorescence analysis to detect intravenously-administered human IgG in formaldehyde-fixed rhesus tissue sections (Schneider et al, 2017).
Antibody First Published
Schneider et al. Long-term direct visualization of passively transferred fluorophore-conjugated antibodies. J Immunol Methods. 2017 Nov;450:66-72 PMID:28780040
Note on publication
This antibody is from the laboratory of Keith Reimann, DVM, Beth Israel Deaconess Medical Center.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
