Cat. No.
MABL-1945
Application
WB, ELISA, purification
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
H15A43
From
Recombinant Antibody
Specificity
This antibody is a recombinant alternative to another anti-human IgA antibody HISA43. It is specific to heavy chain of human IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It reacts with constant domain 3 (CD3) of both subclasses IgA1 and IgA2 of the human IgA heavy chain.
Alternative Names
IGHA1; Immunoglobulin alpha; Immunoglobulin heavy constant alpha 1; Ig alpha-1 chain C region; Ig alpha-1 chain C region BUR; Ig alpha-1 chain C region TRO; HISA43; HISA-43
UniProt
P01876
Immunogen
The original antibody was generated by immunizing BALB/c mice with human IgA myeloma protein or human parotid saliva.
Application Notes
The specificity of this antibody to the CH3 domain of human IgA was determined using western blotting (PMID: 3093370). This recombinant antibody can be used to determine sIgA in a sample using ELISA (PMID:1872572). It can be used for the affinity purification of IgA from serum (PMID: 16395264).
Antibody First Published
Biewenga et al. Production and characterization of pepsin fragments of human IgA1 to determine domain-specificity of monoclonal anti-IgA antibodies. Immunology. (1986); 59(1):153-8. PMID:3093370
Note on publication
Describes the generation of a similar antibody to H15A43.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
