Cat. No.
MABL-1946
Application
functional assay, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AMS9- 9.1.1.1
From
Recombinant Antibody
Specificity
This antibody is specific for IgD (Igh-Ca haplotype).
Alternative Names
IgD
UniProt
Immunogen
This antibody was raised by immunizing C57BL/6 mice with spleen cells.
Application Notes
PI- cells were used in an flow cytometry experiment using this antibody (Stall and Loken, 1984; pmid:6690619). A competitive binding assay on BALB/c mouse spleen cells was preformed using a radiolabelled version of this antibody (May et al, 1990; pmid:1691764). A cytotoxicity assay was preformed on mouse lymph node cells using this antibody. The antibody was 62,5 fold less toxic than ricin (May et al, 1990; pmid:1691764). BALB/c mice were given this antibody as a preventive treatment for chronic TMA- and DNCB-induced CHS (Nguyen, 2018; pmid:30218740). ELISA was preformed on TMA- specific IgM, IgG and IgE in mouse serum with this antibody (Nguyen, 2018; pmid:30218740). Fresh mouse spleen cells were used in flow cytometry using this antibody (Nguyen, 2018; pmid:30218740). B cells from mice were used for immunofluorescence with this antibody (Pereira et al, 2009; pmid:19597478). Horizontal 5 um sections of mice were used for immunohistochemistry using this antibody (Zuercher et al, 2002; pmid:11823512).
Antibody First Published
Stall et al. ALLOTYPIC SPECIFICITIES OF MURINE IgD AND IgM RECOGNIZED BY MONOCLONAL ANTIBODIES J Immunol. 1984 Feb;132(2)
Note on publication
Hybridomas generated from mice immunized with allotype and H-2-incompatible spleen cells were screened by flow cytometry
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
