Cat. No.
MABL-2062
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
UFKA-20
From
Recombinant Antibody
Specificity
This antibody binds human IL-2. In particular, the antibody binds IL-2 dorsolaterally, with an angle of 55° counterclockwise to the vertical axis, contacting the C- and B-helices of IL-2 and thereby forming a “clamp” around the C-helix. The antibody engages IL-2 residues D84, N88, V91, E61, E68, and K64.
Alternative Names
IL2; IL-2; TCGF; Interleukin-2; T-cell growth factor; Aldesleukin
UniProt
P60568
Immunogen
The original antibody was generated immunizing Balb/c mice with human IL-2.
Application Notes
This antibody binds to Interleukin-2, a protein produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/UFKA-20 complex shows an improved selectivity towards regulatory T cells compared to IL-2 alone. IL-2/UFKA-20 can recognize the receptor IL of regulatory T cells on the base of the presence of CD25, therefore called CD25 bias. IL-2/UFKA-20 complex is able to efficiently stimulate CD25+ Treg cells in mice and rhesus macaques in vivo as well as in humans ex vivo.
Antibody First Published
Karakus et al. Receptor-gated IL-2 delivery by an anti-human IL-2 antibody activates regulatory T cells in three different species. Sci Transl Med. 2020 Dec 16;12(574):eabb9283. PMID:33328333
Note on publication
The authors identified a anti-human IL-2 antibody which was particularly efficient in delivering IL-2 to cells expressing trimeric IL-2Rs. Further IL-2/UFKA-20 complex, crystal structure, mechanism of action are described.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
