Cat. No.
MABL-2063
Application
inhibit, neutralize, therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
20A10
From
Recombinant Antibody
Specificity
This antibody binds a conformational epitope which can also be formed or mimicked by the human IL-20 derived peptide comprising amino acids 101-PDHYTLRKISSLANSFLT-118. It also cross reacts with mouse IL-20. IL-20 is a pro-inflammatory and angiogenic cytokine mainly secreted by monocytes, granulocytes, dendritic cells and skin keratinocytes that plays crucial roles in immune responses, regulation of inflammatory responses, hemopoiesis, as well as epidermal cell and keratinocyte differentiation. IL-20 shows effects in several inflammatory diseases, such as rheumatoid arthritis, atherosclerosis, cancer, and liver fibrosis via regulating cytokines and chemokines. IL-20 acts on renal cells and contributes to inflammation, fibrosis, and apoptosis through its receptors IL-20R1, IL-20R2, and IL-22R1.
Alternative Names
IL20; Interleukin-20; ZCYTO10; Cytokine Zcyto10
UniProt
Q9NYY1
Immunogen
The original antibody was isolated from the serum of patients suffering from APECED (Autoimmune polyendocrinopathy candidiasis epidermal dysplasia, also called Autoimmune polyendocrinopathy type 1 (APS1) using a LIPS assay.
Application Notes
This antibody can bind a fusion protein of human IL-20 with Gaussia luciferase in a GLuc-sandwich ELISA. This antibody was capable of neutralizing recombinant IL-20 and IL-20 receptor mediated STAT3 activation and rhIL-20 and IL-20 receptor mediated induction of KZ136-NLuc Reporter Gene. The in vivo therapeutic effect of this antibody was studies using an ear inflammation assay in 8 weeks old C57BL/6J mice by intradermal injection of human cytokine IL-20. This antibody was successful in neutralizing the injected human IL-20 in a mouse model (US20170189524). The IgG4 version of this antibody binds human IL-20 with a very high binding affinity of Kd= 91fM (PMID: 27426947).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
