Cat. No.
MABL-2064
Application
inhibit, therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
7E
From
Recombinant Antibody
Specificity
This antibody binds human Interleukin-20 (IL-20). IL-20 is a pro-inflammatory and angiogenic cytokine mainly secreted by monocytes, granulocytes, dendritic cells and skin keratinocytes that plays crucial roles in immune responses, regulation of inflammatory responses, hemopoiesis, as well as epidermal cell and keratinocyte differentiation. IL-20 shows effects in several inflammatory diseases, such as rheumatoid arthritis, atherosclerosis, cancer, and liver fibrosis via regulating cytokines and chemokines. IL- 20 acts on renal cells and contributes to inflammation, fibrosis, and apoptosis through its receptors IL-20R1, IL-20R2, and IL-22R1.
Alternative Names
IL20; Interleukin-20; ZCYTO10; Cytokine Zcyto10; Mab 7E
UniProt
Q9NYY1
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant human IL- 20.
Application Notes
The binding characterization of this antibody towards human IL-20 was done using ELISA. This antibody inhibits IL-20 induced proliferation of calf pulmonary artery endothelial cells (CPAEs) and human HUVEC cells in vitro (US7786274). The therapeutic efficacy of this antibody was also tested in a collagen-induced arthritis (CIA) rat model. In vivo, treatment with 7E alone or in combination with etanercept significantly reduced the severity of arthritis by decreasing the hind paw thickness and swelling, preventing cartilage damage and bone loss, and reducing the expression of IL-20, IL-1β, IL-6, RANKL, and matrix metalloproteinases (MMPs) in synovial tissue. Selectively blocking IL-20 inhibited inflammation and bone loss in rats with CIA (PMID: 20722035).
Antibody First Published
Hsiang Hsu et al. Interleukin-20 antibody is a potential therapeutic agent for experimental arthritis. Arthritis Rheum. 2010 Nov;62(11):3311-21. PMID:3311-21. PMID:20722035
Note on publication
Determines the in vivo therapeutic efficacy of this antibody in a collagen-induced arthritis (CIA) rat model.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
