Cat. No.
MABL-2070
Application
inhibit, IP, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Mik-beta 1
From
Recombinant Antibody
Specificity
This antibody recognizes and binds the beta chain of the IL-2 receptor and completely inhibits the IL-2 binding to the beta chain. The beta subunit is involved in receptor mediated endocytosis and transduction of the mitogenic signals of IL2.
Alternative Names
CD122; IL-2R; IL2Rβ; IL-2RB; p70-75; p75; Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; High affinity IL-2 receptor subunit beta; Interleukin-15 receptor subunit beta; Mik-β1
UniProt
P14784
Immunogen
The original antibody was generated by immunizing BLAB/c mice with YTS cells, a human NK- like cell line that highly expresses the human IL-2R beta chain as immunogen.
Application Notes
Mik-beta 1 inhibited binding of iodine labelled IL-2 to YTS cells in a dose dependent manner. Mik-beta 1 also successfully immunoprecipitated beta chain of the IL-2R from radio iodinated YTS extract. Biotinylated Mik-beta 1 also efficiently competes with IL-2 for binding to solubilized beta chain. Flow cytometry was used to study the cellular disctribution of beta chain, as determined by mik-beta 1 staining. This antibody completely inhibited IL-2 dependent T cell growth in presence of anti-Tac. Two-color flow cytometric analysis using anti-beta chain mAbs clearly showed that approximately 10% of peripheral blood lymphocytes manifest the beta chain and that this population is mainly composed of Leu-19+ NK cells (PMID: 2467293). Mik beta 1 antibody acts synergistically with humanized anti-Tac to prevent IL-2-induced proliferation of activated T cells (PMID: 8335891). A recombinant single chain immunotoxin of Mik-beta 1 and Pseudomonas derived exotoxin PE40 was selectively cytotoxic for cells expressing high levels of IL-2R beta (p75) subunit (PMID: 1401913).
Antibody First Published
Tsudo et al. Characterization of the interleukin 2 receptor I8 chain using three distinct monoclonal antibodies. Proc Natl Acad Sci U S A. (1989); 86(6): 1982–1986. PMID:2467293
Note on publication
Describes the development and characterization of antibodies against beta chain of IL-2R.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
