Cat. No.
MABL-2108
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Rabbit, Human, Rhesus Monkey
Clone No.
6G4.2.5V11N35A
From
Recombinant Antibody
Specificity
This antibody binds human IL-8, which is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. This antibody cross reacts with rabbit and rhesus monkey IL-8.
Alternative Names
IL8; Interleukin-8; CXCL8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; 6G4.2.5; V11N35A; 6G4V11N35A
UniProt
P10145
Immunogen
The original mouse antibody 6G4.2.5 was generated by immunizing BALB/c mice with recombinant human IL-8. The humanized version is 6G4.2.5 V11N35A was generated by grafting CDRs of mouse parental antibody onto human framework regions.
Application Notes
This antibody is capable of neutralizing the biological activity of wild-type human IL-8. This antibody is reported to inhibit human wild type IL-8, human monomeric IL-8, rabbit IL-8, and rhesus IL-8 mediated neutrophil chemotaxis (US6025158). The binding characterization of this antibody was done using ELISA. The Fab version of this antibody binds human IL-8 with an affinity of 114 pM (US6025158).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
