Cat. No.
MABL-2117
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
RM9A4
From
Recombinant Antibody
Specificity
This antibody is specific for murine IL-9.
Alternative Names
Interleukin-9; IL9; Cytokine P40; T-cell growth factor P40
UniProt
P15247
Immunogen
This antibody was raised by immunising rats with recombinant full-length murine IL-9.
Application Notes
This antibody, when fluorochrome-conjugated, has been used to achieve intracellular IL-9 staining of T cells, analysed using flow cytometry (Chang et al, 2010; Kim et al, 2010; Horka et al, 2012; Gomez-Rodriguez et al, 2016). This antibody has also been used in a sandwich ELISA to measure IL-9 secretion in supernatants collected from restimulated T cells (Jager et al, 2009).
Antibody First Published
Jager et al. Th1, Th17, and Th9 Effector Cells Induce Experimental Autoimmune Encephalomyelitis with Different Pathological Phenotypes J Immunol. 2009 Dec 1;183(11):7169-77. PMID:19890056
Note on publication
Describes the use of this antibody in ELISA analysis to measure cytokine secretion.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
