Cat. No.
MABL-2118
Application
Blocking, IP
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AH9R2
From
Recombinant Antibody
Specificity
This antibody binds specifically to the human IL-9 receptor alpha chain.
Alternative Names
Interleukin-9 receptor; IL9R; CD129
UniProt
Q01113
Immunogen
This antibody was raised by immunising DBA/2 mice with P815 mastocytoma cells transfected with human IL-9R cDNA.
Application Notes
This antibody has been used to immunoprecipitate human IL-9R expressed by murine BW5147 cells transfected with hIL-9R-encoding cDNA (Demoulin et al, 1996). This antibody binds to and blocks the human IL-9Ra, and displays intermediate inhibitory activity; in a TS1H9RA3 proliferation assay, half-maximal inhibition of 100 pg/ml human IL-9 requires 10 mg/ml of this antibody (de Smedt et al, 2000). Equally, treatment with this antibody partially reduces the cellularity of human thymocytes in chimeric human-mouse FTOC, with no effect on murine thymocytes (de Smedt et al, 2000).
Antibody First Published
Demoulin et al. A single tyrosine of the interleukin-9 (IL-9) receptor is required for STAT activation, antiapoptotic activity, and growth regulation by IL-9. Mol Cell Biol. 1996 Sep;16(9):4710-6. PMID:8756628
Note on publication
Describes the original generation of this antibody, and its use in immunoprecipitation analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
