Cat. No.
MABL-2127
Application
Blocking, IP, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
3E 12
From
Recombinant Antibody
Specificity
This conformation-dependent antibody binds to the murine common gamma-chain, shared by the IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 receptor complexes. It recognises an epitope located in a membrane-proximal portion of the common gamma-chain, near Arg-197 of domain 2. This antibody does not cross-react with COS7 cells transfected with rat common gamma-chain cDNA (Olosz et al, 2002).
Alternative Names
CD132; IL2R gamma C; common gamma chain; common y chain; gamma chain; IL-2 receptor gamma chain; Interleukin-2 receptor subunit gamma; Cytokine receptor common subunit gamma; Il2rg; IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG; gammaC; p64
UniProt
P34902
Immunogen
This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing murine cytoplasmic-tailless IL-2R common gamma-chain (YB2/0-myt).
Application Notes
This antibody has been used in flow cytometric analysis of common gamma-chain surface expression by IL-7-stimulated T cells (Goh et al, 2017) and COS7 monkey kidney cells (Olosz et al, 2002). This antibody has also been used to immunoprecipiate murine common gamma-chain-containing complexes from COS7 cell extracts (Olosz et al, 2002). Additionally, as this antibody is conformationally sensitive, it has been used to confirm the correct folding of soluble murine common gamma chain mutants (Meißner et al, 2001). This antagonistic antibody is most effective at inhibiting IL-4 bioactivity (Malek et al, 1998). Treatment of recipient mice with this antibody, along with another common gamma-chain-blocking MAb (4G3), significantly prolongs islet allograft survival (Li et al, 2000). Indeed, blocking the common gamma chain with this antibody has been shown to induce apoptotic cell death of BAF-B03 cells and primary anti-CD3 activated T lymphoblasts in vitro, and of alloreactive T cells in vivo (Li et al, 2000).
Antibody First Published
He et al. Expression and function of the gamma c subunit of the IL-2, IL-4, and IL-7 receptors. Distinct interaction of gamma c in the IL-4 receptor. J Immunol. 1995 Feb 15;154(4):1596-605. PMID:7530740
Note on publication
Describes the original generation of this antibody, and its use in determining murine common gamma-chain lymphoid cell distribution.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
