Cat. No.
MABL-2172
Application
SPR, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2G5
From
Recombinant Antibody
Specificity
2G5 is specific for IRTA5; it does not bind IRTA1, IRTA2, IRTA3, or IRTA4.
Alternative Names
CD307a; Immune Receptor Translocation-Associated Protein 5; Fc Receptor Like 1; FCRL1; FcR-like protein 1; Fc receptor homolog 1; FcRH1
UniProt
Q96LA6
Immunogen
Mice of the HCo7 HuMab Mouse(R) strain were immunized with purified recombinant IRTA5 fusion protein. Mouse splenocytes, isolated from the HuMab mice, were fused with PEG to a mouse myeloma cell line. The resulting hybridomas were then screened for the production of antigen-specific antibodies. The 2G5 clone was selected from this.
Application Notes
SPR was used to determine the Kd of 2G5 binding to IRTA5 as 0.028 x 10^-9 M. Using flow cytometry 2G5 was shown to be specific for IRTA5; it does not bind IRTA1, IRTA2, IRTA3, or IRTA4. Flow cytometry also showed that 2G5 directed against human IRTA-5 binds to CD 19+ B cells and the B-cell tumor lines Karpas 1106P, SU-DHL-4, Granta 519, and L-540. 2G5 does not bind CD3+ peripheral blood T cells, CD 1A+ peripheral blood dendritic cells, CD14+ peripheral blood monocytes, or CD56+ peripheral blood NK cells.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
