Cat. No.
MABL-2173
Application
IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
H116
From
Recombinant Antibody
Specificity
This antibody is specific for an unknown autoantigen (still under investigation, as of 2016), expressed in islet beta cells (also referred to as intraislet).
Alternative Names
Unknown islet β-cells autoantigen; unknown intraislets autoantigen
UniProt
Immunogen
The original antibody was generated from a B lymphocyte islet-infiltrate of a diabetes- resistant but insulitis-prone (8.3-NODxNOR) F1 mouse.
Application Notes
This antibody recognizes antigenic determinants selectively expressed in islet β-cells of the pancreas, as determined by immunofluorescence (IF) staining; its staining pattern mimics the pattern obtained with anti-insulin antibodies. Furthermore, this antibody was shown to be cross-reactive with stomach and kidney cryosections of NOD.RAG-2−/− mice (Carrillo et al., 2005; PMID: 15616012). This antibody was also used for tissue immunostaining of pancreatic cryosections from NOD.RAG-2−/− mice (Carrascal et al., 2016; PMID: 26639224).
Antibody First Published
Carrillo et al. Islet-infiltrating B-cells in nonobese diabetic mice predominantly target nervous system elements Diabetes. 2005 Jan;54(1):69-77. doi: 10.2337/diabetes.54.1.69 PMID:15616012
Note on publication
The original publication describes the generation of hybridoma cell lines of islet- infiltrating B-cells from mice with type 1 diabetes and diabetes-resistant mice, and the characterization of the tissue specificity of the antibodies produced by these hybridomas.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
