Cat. No.
MABL-2242
Application
inhibition, IP, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Bovine, Human
Clone No.
C7
From
Recombinant Antibody
Specificity
The antibody recognizes the ligand binding domain. It binds to the bovine LDL-R, the human LDL-R but it does not cross react with the LDL-Rs of rat, mouse, Chinese hamster, rabbit and dog. The LDL receptor is a cell surface protein that binds LDL, the major cholesterol-transport protein in plasma, and mediates its endocytosis.
Alternative Names
LDLR; Low-density lipoprotein receptor; IgG-C7
UniProt
P01130
Immunogen
The original antibody was produced by immunization of BALB/C mice with the extra cellular domain of the bovine LDL-R.
Application Notes
The antibody (IgG2b) was employed for the staining of the LDL receptor in normal fibroblast. The antibody competed with LDL for binding to the LDL receptor of intact fibroblast; complete inhibition was achieved at 37 C but not at 4 C (Beisiegel et al., 1981; PMID: 6271765). The antibody detected LDL receptor from human fibroblasts and bovine adrenal cortex by western blot analysis (Beisiegel et al., 1982; PMID: 6290495). The LDL receptor was immunoprecipitated using this antibody (Davis et al., 1987; PMID: 3494949). This antibody was used for detection of LDL receptor expressed on Chang and CHO cells by flow cytometry (Holst et al., 2001; PMID: 11781697) (Ranheim et al., 2006; PMID:16740646).
Antibody First Published
Beisiegel et al. Monoclonal Antibodies to the Low Density Lipoprotein Receptor as Probes for Study of Receptor-mediated Endocytosis and the Genetics of Familial Hypercholesterolemia J Biol Chem. 1981 Nov 25;256(22):11923-31. PMID:6271765
Note on publication
The paper describes the generation and characterization of the antibody. The antibody was used as a probe for the study of the cellular and genetic factors involved in receptor-mediated endocytosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
