Cat. No.
MABL-2279
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
06ad P. aeruginosa
Clone No.
S20
From
Recombinant Antibody
Specificity
S20 binds to the polysaccharide (PS) portion of the lipopolysaccharide O side chain of a common pathogenic serogroup of P. aeruginosa, 06ad. The antibody also binds Fisher Devlin immunotype 1 P. aeruginosa, (considered part of the 06 serogroup) but there is no cross-reactivity with LPSs from other P. aeruginosa serotypes (strains IATS 011, IATS 016, 170003, and PAO1 Holloway). P. aeruginosa is a human pathogen, which poses a serious risk to individuals with weak immune systems. LPS is a serotypic marker present on the surface of the bacterium.
Alternative Names
lipopolysaccharide; lipopolysaccharide O side chain
UniProt
Immunogen
Ig-inactivated transgenic mice possessing human heavy and light Igs (strain XMG2) were immunized with heat-killed 06ad P. aeruginosa. The supernatants from the derived hybridomas were screened for IgG mAb production against the 06ad high molecular weight LPS O-side-chain using ELISA.
Application Notes
S20 human MAb is highly opsonic (complement-dependent) for the uptake and killing of live and heat-killed P. aeruginosa by fresh human PMN ( polymorphonuclear leukocytes). BALB/c mice rendered neutropenic challenged with 06ad P. aeruginosa saw 100% protection from mortality. S20 can also be used to detect 06ad LPS by ELISA.
Antibody First Published
Hemachandra Human Monoclonal Antibodies against Pseudomonas aeruginosa Lipopolysaccharide Derived from Transgenic Mice Containing Megabase Human Immunoglobulin Loci Are Opsonic and Protective against Fatal Pseudomonas Sepsis Infect Immun. 2001 Apr;69(4):2223-9 PMID:11254577
Note on publication
Describes the generation of a human mAb against 06ad P. aeruginosa LPS, its binding properites and its protective potential.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
