Cat. No.
MABL-2280
Application
IB, WB
Isotype
Engineer antibody
Species Reactivity
Rabbit, Rat, Human, Mouse
Clone No.
5A6
From
Recombinant Antibody
Specificity
This antibody is specific for LRP1, and reacts with an epitope in the 85kDa light beta chain (LRP-85 subunit).
Alternative Names
low density lipoprotein receptor-related protein; LRP-85; LRP1; alpha-2- macroglobulin receptor; A2MR; APR; apolipoprotein E receptor; APOER; cluster of differentiation 91; CD91; LRP-1; 85 kDa light chain of LRP
UniProt
Q07954
Immunogen
This antibody was raised by immunising mice with human LRP, purified by affinity chromatography from detergent placenta extracts.
Application Notes
The specificity of this antibody has been confirmed in immunoblotting experiments (Strickland et al, 1990) and Western blot analysis (Mikhailenko et al, 2001). This antibody, when radiolabelled, has been used in cellular internalisation assays using CHO cells transfected with various receptors (Mikhailenko et al, 2001) and HT1080 fibrosarcoma cells transfected with MT1-MMP (Rozanov et al, 2004). This antibody has also been used in immunoblotting analysis of murine brain samples (Billings et al, 2007), murine vascular smooth muscle cells (Lehti et al, 2009), murine bone-marrow-derived macrophages (Chen et al, 2017) and human amnion epithelium and mesoderm samples (Lee et al, 2010). Additionally, it has been used in Western blot analysis of the CXCR3:LRP1 complex from human glioma cells and coronary artery smooth muscle cells (Boye et al, 2017). This antibody does not inhibit LRP-mediated internalisation of activated alpha2-macroglobulin, nor affect the binding of activated alpha2-macroglobulin to immobilised LRP (Mikhailenko et al, 2001).
Antibody First Published
Strickland et al. Sequence identity between the alpha 2-macroglobulin receptor and low density lipoprotein receptor-related protein suggests that this molecule is a multifunctional receptor. J Biol Chem. 1990 Oct 15;265(29):17401-4. PMID:1698775
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity in immunoblotting experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
