Cat. No.
MABL-2281
Application
IB, WB
Isotype
Engineer antibody
Species Reactivity
Rabbit, Rat, Human, Mouse
Clone No.
8B8
From
Recombinant Antibody
Specificity
This antibody is specific for LRP1, and reacts with an epitope in the 85kDa light beta chain (LRP-85 subunit).
Alternative Names
low density lipoprotein receptor-related protein; LRP-85; LRP1; alpha-2- macroglobulin receptor; A2MR; APR; apolipoprotein E receptor; APOER; cluster of differentiation 91; CD91; LRP-1; 85 kDa light chain of LRP
UniProt
Q07954
Immunogen
This antibody was raised by immunising mice with human LRP, purified by affinity chromatography from detergent placenta extracts.
Application Notes
The specificity of this antibody has been confirmed in immunoblotting experiments (Strickland et al, 1990). This antibody has been used in Western blot analysis of HEK-293 cells transfected with human full-length LRP1 fused to EGFP, to confirm fusion protein expression (Laatsch et al, 2012). Additionally, this antibody has been used in Western blot analysis to measure LRP1 protein expression levels, both in human vascular smooth muscle cells (Costales et al, 2015; Sendra et al, 2008) and human monocyte-derived macrophages (Llorente-Cortés et al, 2007).
Antibody First Published
Strickland et al. Sequence identity between the alpha 2-macroglobulin receptor and low density lipoprotein receptor-related protein suggests that this molecule is a multifunctional receptor. J Biol Chem. 1990 Oct 15;265(29):17401-4. PMID:1698775
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity in immunoblotting experiments.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
