Cat. No.
MABL-2444
Application
neutralizing, ELISA
Isotype
Engineer antibody
Species Reactivity
Bothrops atrox
Clone No.
M85
From
Recombinant Antibody
Specificity
The original antibody is specific for the myotoxic protein of Bothrops atrox.
Alternative Names
Myotoxin I; svPLA2 homolog; Phospholipase A2 homolog 1
UniProt
Q6JK69
Immunogen
The original antibody was generated by immunizing a two-year-old adult male L. glama with increasing doses of B. atrox venom, followed by a VHH library construction and panning against partially purified hemorrhagic and myotoxic proteins.
Application Notes
The specificity of the original format of the antibody was confirmed by ELISA analysis. The original antibody was able to decrease myonecrosis induced by the whole venom, as evaluated by the CK activity levels in plasma (Calderon et al., 2020; PMID: 32457735).
Antibody First Published
Calderon et al. Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom Front Immunol. 2020 May 7:11:655. doi: 10.3389/fimmu.2020.00655. eCollection 2020. PMID:32457735
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
