Cat. No.
MABL-2448
Application
Agglutination reaction, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
NNA7
From
Recombinant Antibody
Specificity
This antibody recognizes the human N-type blood-group antigen (MNS blood group) comprised of the N-terminal glycopeptide of glycophorin A (GPA). Glycophorin A is the major sialoglycoproteins and intrinsic membrane protein of the human erythrocyte which bears the antigenic determinants for the MN and Ss blood groups.
Alternative Names
CD235a; Glycophorin-A; GYPA; GPA; PAS-2; N sialoglycoprotein; Sialoglycoprotein alpha; Glycophorin A N antigen; MNS blood group antigen; glycopeptide N blood-group antigen; red blood cell antigen; N-type glycophorin A; N72
UniProt
P02724
Immunogen
The original antibody was isolated from light-chain cDNA libraries constructed from mice immunized with N-type glycophorin A. These were inserted into a recombinant pComb3H vector containing the N92 Fd fragment. The N92 Fd fragment: light-chain libraries were panned on N-type glycophorin A or NN red cells.
Application Notes
This antibody is a synthetic clone derives from the parental clone N72 Fab. This antibody is capable of reacting with N-type glycophorin A and NN red cells in an ELISA and an agglutination assay (PMID: 10220261). This antibody is capable of detecting glycophorin A of N-type and glycophorin B in a western blot assay (PMID: 19833240; 9574545). This antibody was also used in the immunofluorescence staining of transfected CHO cells expressing recombinant N-type GPA (PMID: 14962308).
Antibody First Published
Czerwinski et al. A molecular approach for isolating high-affinity Fab fragments that are useful in blood group serology. Transfusion. 1999 Apr;39(4):364-71. PMID:10220261
Note on publication
This paper describes the use of light chain shuffling to generated mutant parental clones with higher affinity for glycophorin A.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
