Cat. No.
MABL-2451
Application
ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
E1
From
Recombinant Antibody
Specificity
Gangliosides are glycosphingolipids expressed on the cell surface membranes of most mammalian cell types. In ELISA experiments E1 showed a highly-restricted binding specificity, detecting only GM2 (not GM1 or GM3). The antibody recognises only the N-acetylneuraminic acid deratives of gangliosides; no N-glycolylneuraminic acid derivatives were recognised.
Alternative Names
UniProt
Immunogen
The antibody was generated by injecting BALB/c mice with GM2 ganglioside incorporated into liposomes.
Application Notes
ELISA demonstrated E1 has a highly-restricted binding specificity, binding only to GM2. IHC studies showed positivity with E1 in primary lung carcinomas, but no reactivity with corresponding normal tissues (Alfonso et al, 1995).
Antibody First Published
Alfonso et al, 1995. T Cell-Independent Cell Response to Self- Monosialogangliosides: Primary Response Monoclonal Antibodies Hybridoma. 1995 Jun;14(3):209-16 PMID:7590782
Note on publication
Describes the original generation of E1 and its characterisation through ELISA and IHC.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
