Cat. No.
MABL-2452
Application
Blocking, infectious disease, ELISA
Isotype
Engineer antibody
Species Reactivity
P. aeruginosa
Clone No.
RS2-1G9
From
Recombinant Antibody
Specificity
Binds 3-oxo-C12-HSL with a Kd of 150 nM
Alternative Names
HSL;AHL
UniProt
Immunogen
The 4-methoxyphenyl amide AHL analog.
Application Notes
This antibody, originally raised in mice, binds to several homoserine lactones from P. aeruginosa. Homoserine lactones (HSL) are signalling molecules in bacteria used for quorum sensing, required for successful estabilshment of infecion. RS2-1G9 interferes with quorum sensing and was shown to protect murine bone marrow derived macrophages, from the cytotoxic P. aeruginosa quorum sensing signalling molecule N-3-oxo-dodecanoyl-homoserine lactone (PMID: 18304641).
Antibody First Published
Kaufmann et al. 2006 Antibody Interference with N-Acyl Hosoerine Lacone- Mediated Bacterial Quorum Sensing. Journal of the American Chemical Society. 2006; 128 (9): 2802-2803 PMID:16506750
Note on publication
Describes the design of HSL-analogous haptens used for the immunization of mice for hybridoma production of the 1G9 antibody as well as charcterization of the inhibibition of quorum sensing by the produced antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
