Cat. No.
MABL-2453
Application
WB, FC, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Hamster, Rabbit, Human
Clone No.
8C11
From
Recombinant Antibody
Specificity
This antibody recognizes human, rabbit and hamster extracellular domain of N-cadherin. It does not cross react with this protein in mouse, rat cow and pig. N-cadherin is a calcium-dependent cell adhesion protein.
Alternative Names
CD325; Cadherin-2; CDw325; Neural cadherin; n-cadherin; ncadherin
UniProt
P19022
Immunogen
This antibody was raised by immunizing mice with an extracellular domain of the recombinant human N-cadherin.
Application Notes
8C11 antibody inhibits cells' motility, as it blocks extracellular domain of N-cadherin which is responsible for this function (Kim et al., 2000). This antibody has also been widely used in the detection and analysis of N-cadherin. For instance, it was utilized to identify this protein in A549 cells via immunofluorescence (Shintani et al., 2011). Another group used successfully 8C11 to analyze the levels of N-cadherin expression via Western blot (Chen et al., 2015). Finally, this antibody was used to label N- cadherin in tumor tissues of a metastasis model via immunohistochemistry (Li et al., 2019).
Antibody First Published
Kim et al. N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility. J Cell Biol. 2000 Dec 11;151(6):1193-206. PMID:11121435
Note on publication
This article describes the generation and characterization of the antibody 8C11.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
