Cat. No.
MABL-2454
Application
IP, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
OX-133
From
Recombinant Antibody
Specificity
This antibody recognises cysteine-boud N-ethylmaleimide in a sequence-independent manner. It has no affinity for unmodified peptides or peptides modified with other alkylating agents such as iodoacetamide.
Alternative Names
NEM cysteine; OX133
UniProt
Immunogen
Purified MBP-gp120 (HIV-1) reduced using tris(2-carboxyethyl)phosphine (TCEP, 2.5 mM) for 20 minutes and alkylated using NEM (5 mM solubilized in PBS) for 30 minutes.
Application Notes
OX133 detects NEM-modified proteins and can be used as an affinity matrix to purify NEM-modified proteins from cell lysates. OX133 does not cross react with any other alkylating agent, making it a highly selective reagent for the purification of NEM-labeled proteins and potentially peptides for mass spectrometry-based analysis.
Antibody First Published
Holbrook et al. OX133, a monoclonal antibody recognizing protein-bound N- ethylmaleimide for the identification of reduced disulfide bonds in proteins MAbs. 2016 May-Jun;8(4):672-7 PMID:26986548
Note on publication
Describes the generation and characterisation of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
