Cat. No.
MABL-2455
Application
crystallization, functional assays, HA, HPTLC, in vivo, therapeutic, TLC, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
14F7
From
Recombinant Antibody
Specificity
This antibody is specific for GM3(NeuGc). It does not cross-react with other N-glycolyl or N- acetyl gangliosides nor sulfated glycolipids.
Alternative Names
GM3(NeuGc) ganglioside
UniProt
Immunogen
The original antibody was generated by immunizing Balb/c mice with GM3(NeuGc) ganglioside hydrophobically conjugated with human very-low-density lipoproteins (VLDL).
Application Notes
The original format of this antibody (mouse IgG1) was generated and evaluated. In an ELISA and TLC assay, it was shown to specifically bind NeuGcGM3. In an IHC assay, it was shown to react with all ductal infiltrating breast carcinoma and melanoma tissues tested but not with samples from lung carcinoma of different histological types or nervous system tumors. Some benign lesions of the breast (such as breast fibrocystic and breast fibroadenoma) showed a positive staining to extracellular secretion.Furthermore, it stained the extracellular secretion of breast glands and mucus cells from the small intestine and large intestine (Carr et al., 2000; PMID: 10952412). The original format of this antibody successfully agglutinated horse erythrocytes, but not those from dog, monkey, or human origin, 4°C and 37°C in an HA assay.
Antibody First Published
Carr et al., A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors. Hybridoma. 2000 Jun;19(3):241-7. doi: 10.1089/02724570050109639. PMID:10952412
Note on publication
The original publication details the generation of the 14F7 murine monoclonal antibody, an IgG1 isotype that specifically binds to GM3(NeuGc) ganglioside and was shown to recognize antigens in human breast and melanoma tumors in immunohistochemical studies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
